【摘要】 【目的】益生菌粘附于肠道上皮细胞上是它的一种益生作用。本研究通过体内外实验,分析嗜酸乳杆菌NCFM对粘附相关基因的影响。【方法】利用GO(Gene Ontolog)分类筛选Human Genome U133 Plus 2.0Array基因表达谱芯片中的粘附相关基因,通过体外Caco-2细胞培养模型和体内小鼠粘附模型,采用Real-time PCR方法对粘附相关基因进行验证分析。【结果】经NCFM作用后,12个粘附相关基因呈上调表达。利用Real-time PCR验证,12个基因在体内和体外经嗜酸乳杆菌NCFM作用后亦均同样为上调表达,其中CCL2基因上调表达最为明显。【结论】经体内外研究表明,嗜酸乳杆菌NCFM粘附肠上皮细胞后能够引起宿主粘附相关基因出现特定表达变化,为今后深入揭示其粘附作用提供必要基础。更多还原

【Abstract】 [Objective]Adhering to the intestinal epithelial cells is one of the beneficial functions exerting by probiotics.We tested and verified the expression of adhesion-related genes in vivo and in vitro to understand the influence from Lactobacillus acidophilus NCFM on host cells.[Method]We selected the adhesion-related genes through GO(gene ontology) category from the Human Genome U133 Plus 2.0 Array analysis.These genes were verified by in vitro Caco-2 cells culture model and in vivo mouse model using Real-time PCR method.[Results] After L.acidophilus NCFM adhering to Caco-2,we found that 12 adhesion-related genes were up-regulated.The up-regulation was confirmed in vivo and in vitro by Real-time PCR assay.Among them,the up-regulated expression of chemokine(C-C motif) ligand 2(CCL2) gene was the most distinguished one.[Conclusion]Our research showed that L.acidophilus NCFM,adhering to Caco-2 cells,could cause the differential expression of the host adhesion-related genes.Results of this study seemed to provide some useful data for further revealing its effect on intestinal epithelial cells.更多还原