节点文献
棉秸秆降解高温菌株的筛选及产酶分析
Isolation and characterization of four cellulose-decomposing thermophilic fungal strains from Xinjiang
【摘要】 从新疆地区分离具有降解棉秸秆纤维素功能的菌株,得到4株耐高温真菌(50°C)。纤维素酶学性质分析表明,该4株菌的纤维素酶具有良好的耐酸性(最适pH为4.5)和耐高温性(最高达60°C)。以羧甲基纤维素钠(CMC-Na)、微结晶纤维素、棉花、滤纸、淀粉、果胶为底物测定酶活力,滤纸酶活力(FPA)最高达2.63 U/mL、淀粉酶活力最高达6.17 U/mL、果胶酶活力最高达5.86 U/mL。4株真菌酶学特性分析表明,该系列菌株在秸秆生物质利用方面有很大的应用潜力。
【Abstract】 Four thermophilic fungal strains producing cellulase(50 °C) were isolated from the compost of cotton residue in Xinjiang,having the ability of acid-resistant(the optimal pH is 4.5) and temperature-resistant(the highest temperature is 60 °C).The enzyme activities were measured using multiple substrates,such as sodium carboxy methyl cellulose(CMC-Na),microcrystalline cellulose,cotton,filter paper,starch and pectin.The highest filter paper enzyme activity(FPA) is 2.63 U/mL,the amylase activity is 6.17 U/mL and the pectinase activity is 5.86 U/mL.It was showed that these fungal strains and their secreted enzymes have great potential on cellulolytic biomass(cotton residue) degradation and utilization.
【Key words】 Temperature; Cellulolytic fungi; Cellulase; Cotton residue;
- 【文献出处】 微生物学通报 ,Microbiology China , 编辑部邮箱 ,2011年02期
- 【分类号】S182
- 【被引频次】15
- 【下载频次】410