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棉秸秆降解高温菌株的筛选及产酶分析

Isolation and characterization of four cellulose-decomposing thermophilic fungal strains from Xinjiang

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【作者】 周亚飞詹发强侯新强赵忠润崔卫东龙宣杞王炜

【Author】 ZHOU Ya-Fei1,2 ZHAN Fa-Qiang2 HOU Xin-Qiang2 ZHAO Zhong-Run1,2 CUI Wei-Dong2 LONG Xuan-Qi2 WANG Wei2(1.College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832003,China)(2.Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi,Xinjiang 830091,China)

【机构】 石河子大学生命科学院新疆农业科学院微生物应用研究所

【摘要】 从新疆地区分离具有降解棉秸秆纤维素功能的菌株,得到4株耐高温真菌(50°C)。纤维素酶学性质分析表明,该4株菌的纤维素酶具有良好的耐酸性(最适pH为4.5)和耐高温性(最高达60°C)。以羧甲基纤维素钠(CMC-Na)、微结晶纤维素、棉花、滤纸、淀粉、果胶为底物测定酶活力,滤纸酶活力(FPA)最高达2.63 U/mL、淀粉酶活力最高达6.17 U/mL、果胶酶活力最高达5.86 U/mL。4株真菌酶学特性分析表明,该系列菌株在秸秆生物质利用方面有很大的应用潜力。

【Abstract】 Four thermophilic fungal strains producing cellulase(50 °C) were isolated from the compost of cotton residue in Xinjiang,having the ability of acid-resistant(the optimal pH is 4.5) and temperature-resistant(the highest temperature is 60 °C).The enzyme activities were measured using multiple substrates,such as sodium carboxy methyl cellulose(CMC-Na),microcrystalline cellulose,cotton,filter paper,starch and pectin.The highest filter paper enzyme activity(FPA) is 2.63 U/mL,the amylase activity is 6.17 U/mL and the pectinase activity is 5.86 U/mL.It was showed that these fungal strains and their secreted enzymes have great potential on cellulolytic biomass(cotton residue) degradation and utilization.

【关键词】 高温纤维素分解菌酶活力棉花秸秆
【Key words】 TemperatureCellulolytic fungiCellulaseCotton residue
【基金】 国家公益性行业(农业)专项(No.2009-2011);自治区高技术研究发展项目(No.2009-2011)
  • 【文献出处】 微生物学通报 ,Microbiology China , 编辑部邮箱 ,2011年02期
  • 【分类号】S182
  • 【被引频次】15
  • 【下载频次】410
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