【摘要】 目的:探讨人重组α干扰素(rhIFN-α)及联合应用顺铂对骨肉瘤细胞(MG-63)凋亡的影响。方法:将rhIFN-α和顺铂分别和联合作用于MG-63细胞,光镜下观察细胞形态学改变,用MTT检测MG-63细胞增殖抑制情况,流式细胞仪法检测MG-63细胞凋亡情况,Hoechest 33258染色和DNAladder检测细胞凋亡。结果:10 000 IU/ml rhIFN-α对细胞增殖抑制情况较明显(34.12±2.11)%;单独应用顺铂组对细胞增殖抑制率为(24.74±2.22)%;顺铂联合应用干扰素可明显提高MG-63细胞的增殖抑制作用到(69.41±1.72)%、(58.81±2.23)%、(48.23±1.12)%。结论:rhIFN-α对MG-63细胞的增殖抑制效应具有剂量依赖性特点,10 000IU/ml rhIFN-α能明显诱导MG-63细胞凋亡,而且不同剂量的rhIFN-α均能明显促进顺铂诱导的细胞凋亡。提示化疗时联合应用rhIFN-α可提高骨肉瘤细胞对化疗药物的敏感性。更多还原

【Abstract】 Objective:To investigate the effect of combined recombinant human interferon-α(rhIFN-α) with cisplatin on the apoptosis of osteosarcoma cells(MG-63).Methods:We treated the osteosarcoma cells by combining rhIFN-α with cisplatin and the morphological change of the cells were observed by light microscope.The proliferation and apoptosis of osteosarcoma cells were examined respectively by MTT and flow cytometry,and further detection of the apoptosis was carried out with hoechest 33258 staining and DNA ladder solution.Results:Induction by 10 000 IU/ml rhIFN-α could produce evident effect on the ratio of apoptosis(34.12±2.11)% as compared with by single use of cisplatin(24.74±2.22)%,and yet,combined use of rhIFN-α with cisplatin could boost the apoptosis ratio of MG-63 to(69.41±1.72)%,(58.81±2.23)% and(48.23±1.12)%,respectively.Conclusion:The apoptosis ratio of osteosarcoma cells(MG-63) induced by rhIFN-α demonstrated dose dependent.10 000 IU/ml rhIFN-α can significantly induce the apoptosis of MG-63 and different dose of rhIFN-α appears to boost the apoptosis induced by cisplatin,which suggests that combined therapy of cisplatin with rhIFN-α can improve the response of the tumor cells to the medication.更多还原