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Anti-inflammatory Effects and Mechanisms of Usnic Acid

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【Author】 HUANG Zhijun1,2,ZHENG Guohua3,TAOJunyan 3,RUAN Jinlan1(1.College of Pharmacy,Tongji Medical Center,Huazhong University of Science and Technology,Wuhan 430030,China;2.School of Chemical Engineering,Wuhan University of Technology,Wuhan 430070,China;3.School of Pharmacy,Hubei University of Traditional Chinese Medicine,Wuhan 430065,China)

【机构】 College of Pharmacy,Tongji Medical Center,Huazhong University of Science and Technology； School of Chemical Engineering,Wuhan University of Technology；

【摘要】 The anti-inflammatory effect and mechanism of Usnic acid (UA) were explored on lipopoly-saccharide (LPS)-stimulated RAW264.7 cell line.The effects of UA on pro-inflammatory cytokines including tumor necrosis factor-alfa (TNF-α),interleukin-6 (IL-6) and interleukin-1 beta (IL-1β),pro-inflammatory mediators such as nitric oxide (NO),inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were studied by sandwich ELISA,real-time PCR and western blot analyses.Similarly,the effect of UA on anti-inflammatory cytokine interleukin-10 (IL-10) and anti-inflammatory mediator heme oxygenase-1 (HO-1) were also studied following the same methods.Furthermore,nuclear factor-κB (NF-κB) was assayed by immunocytochemistry.The results showed that UA has anti-inflammatory effect by down-regulatinng iNOS,COX-2,IL-1β,IL-6 and TNF-α,COX-2 gene expression through the suppression of NF-κB activation and increasing anti-inflammatory cytokine IL-10 and anti-inflammatory mediator HO-1 production.更多还原

【Abstract】 The anti-inflammatory effect and mechanism of Usnic acid (UA) were explored on lipopoly-saccharide (LPS)-stimulated RAW264.7 cell line.The effects of UA on pro-inflammatory cytokines including tumor necrosis factor-alfa (TNF-α),interleukin-6 (IL-6) and interleukin-1 beta (IL-1β),pro-inflammatory mediators such as nitric oxide (NO),inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were studied by sandwich ELISA,real-time PCR and western blot analyses.Similarly,the effect of UA on anti-inflammatory cytokine interleukin-10 (IL-10) and anti-inflammatory mediator heme oxygenase-1 (HO-1) were also studied following the same methods.Furthermore,nuclear factor-κB (NF-κB) was assayed by immunocytochemistry.The results showed that UA has anti-inflammatory effect by down-regulatinng iNOS,COX-2,IL-1β,IL-6 and TNF-α,COX-2 gene expression through the suppression of NF-κB activation and increasing anti-inflammatory cytokine IL-10 and anti-inflammatory mediator HO-1 production.更多还原

【关键词】 usnic acid； anti-inflammation； western blot analyses； immunocytochemistry；
【Key words】 usnic acid； anti-inflammation； western blot analyses； immunocytochemistry；

【文献出处】 Journal of Wuhan University of Technology(Materials Science Edition) ,武汉理工大学学报(材料科学版)(英文版) , 编辑部邮箱 ,2011年05期

【分类号】R965
【被引频次】6
【下载频次】65

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