【摘要】 目的观察还原型谷胱甘肽和多烯磷脂酰胆碱对高脂喂养SD大鼠非酒精性脂性肝炎的治疗效果,并探讨其可能机制。方法将实验大鼠随机分为正常对照组(n=10)、模型组(n=10)和药物治疗组(还原型谷胱甘肽组和多烯磷脂酰胆碱组,每组10只),通过高脂喂养12周后分别给予治疗组还原型谷胱甘肽和多烯磷脂酰胆碱治疗,8周后处死对照组、模型组和治疗组所有大鼠,观察肝脏组织学变化,并测定血清丙二醛(MDA)、超氧化物歧化酶(SOD)、肿瘤坏死因子(TNF)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、甘油三酯(TG)、总胆固醇(TC)等指标。结果与模型组比较,还原型谷胱甘肽组和多烯磷脂酰胆碱组的大鼠肝脏湿重、内脏脂肪质量、肝指数、肿瘤坏死因子、甘油三酯、总胆固醇、肝脏炎症及纤维化评分显著改善(P<0.01),还原型谷胱甘肽组的丙二醛和超氧化物歧化酶较模型组明显改善(P<0.01)。结论还原型谷胱甘肽和多烯磷脂酰胆碱可促进非酒精性脂肪性肝炎大鼠肝功能的恢复,并且可以改善肝脏组织学。更多还原

【Abstract】 Objective To investigate the effects of reduced glutathione and polyene phosphatidylcholine on nonalcoholic steatohepatisis in high-diet rats,and explore the possible mechanisms.Methods Normal male SD rats were randomly divided into 4 groups: normal control group(N,n=10),model group(M,n=10),reduced glutathione treatment group(GSH,n=10),polyene phosphatidylcholine treatment group(PPC,n=10).All rats of model group(M group) and treatment groups(GSH and PPC groups) were fed with high-fat diet.Treatment groups were given reduced glutathione and polyene phosphatidylcholine at week 12 respectively,and all rats were sacrificed at week 20.The liver steatosis,inflammation and fibrosis were observed by HE stain and Masson stain.Malondialdehyde(MDA),superoxide dismutase(SOD),serum tumor necrosis factor(TNF-α),alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG) and total cholesterol(TC) were measured.Results Compared with model group,liver wet weight,viscera fat quality,TNF-α,TG,liver inflammation and liver fibrosis in treatment groups improved significantly(P<0.01).Compared with model group,MDA and SOD in reduced glutathione group improved significantly(P<0.01).Conclusion Reduced glutathione and polyene phosphatidylcholine are beneficial to the recovery of liver function and can be used to improve nonalcoholic steatohepatitis in rats.更多还原