【摘要】 目的探讨粪便钙卫蛋白(FCP)在腹泻鉴别诊断中的临床应用价值。方法感染性腹泻48例、腹泻型肠易激综合征(D-IBS)30例及正常对照组30例分别留取粪便5~10 g,用ELISA法检测粪便钙卫蛋白含量;同时收集患者病史及临床资料。结果感染性腹泻病人粪便钙卫蛋白检测值(515.21μg/g,n=48)显著高于D-IBS组(35.02μg/g,n=30)和正常对照组(25.18μg/g,n=30)(P<0.001)。D-IBS组和正常对照组之间无明显差异(P>0.05)。感染性腹泻FCP检测值与粪便显微镜下白细胞个数呈正相关(r=0.761)。设定FCP值100μg/g为临界值时判断感染性腹泻的检测敏感性为85.4%特异性为90.0%准确性为87.2%。结论 FCP有望用于感染性腹泻和非感染性腹泻的初步鉴别,具有快速、简便、准确率高、患者依从性好等优点,值得临床推广应用。更多还原

【Abstract】 Objective To explore the clinical value of fecal calprotectin in the differential diagnosis of infectious diarrhea.Methods Thirty people for health examination as controls,forty-eight patients with infectious diarrhea and thirty patients with diarrhea-predominant irritable bowel syndrome(D-IBS) were recruited.Stool sample(5~10 g) was collected in each people to determine fecal calprotectin by an emzyme-linked immunosorbent assay;meanwhile the case history and clinical data were collected simultaneously.Results The fecal calprotectin concentration in infectious diarrhea patients(515.21 μg/g,n=48) was significantly higher than that in the healthy controls(25.18 μg/g,n=30)and the DIBS patients(35.02 μg/g,n=30,P<0.001).There was no statistical significance of calprotectin concentration between D-IBS patients and healthy controls (P>0.05).The level of FCP in infectious diarrhea patients was related with the quantity of fecal leukocytes(r=0.761).When the cut-off limit of fecal calprotectin was set as 100 μg/g,its diagnostic sensitivity of infectious diarrhea was 85.4%,diagnostic specificity was 90.0%,accuracy was 87.2%.Conclusion Fecal calprotectin could be used as a promsing biomarker in the preliminary diagnosis and differential diagnosis of infectious diarrhea and non-infectious diarrhea.It is convenient,economical and worth spreading in clinical works.更多还原