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濒危植物普陀鹅耳枥亲子代遗传多样性的RAPD分析

RAPD analysis of genetic diversity of different progenies from endangered plant Carpinus putoensis

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【作者】 张晓华王正加李修鹏俞慈英陈叶平

【Author】 Zhang Xiaohua1,Wang Zhengjia2,Li Xiupeng3,Yu Ciying4,Chen Yeping4(1.Zhoushan Agriculture and Forestry Bureau,Zhejiang Dinghai 316000;2.Zhejiang A & F University;3.NingboTechnology Extension Center for Forestry and Specialty Forest Products;4.Zhoushan Forestry Institute)

【机构】 浙江省舟山市农林局浙江农林大学浙江省宁波市林特科技推广中心浙江省舟山市林业科学研究所

【摘要】 利用RAPD标记对普陀鹅耳枥亲子代进行了遗传多样性分析,结果表明,普陀鹅耳枥亲子代之间多态性比例(PPL)为4.88%,Nei s基因多样度为0.0135,Shannon多态性信息指数为0.0215,亲、子代间的基因分化系数(Gst)为1.0000,不同世代间的基因流为0.0000。17个样品间的遗传同一性均在0.9634~1.0000之间,而相互之间的遗传距离在0.0000~0.0373之间,表明普陀鹅耳枥的遗传多样性水平低,亲、子代之间保持了非常稳定的遗传关系。

【Abstract】 Genetic diversity of different progenies from endangered plant Carpinus putoensis was analysised by RAPD techniques.Results showed that the proportion of polymorphic loci(PPL) in Carpinus putoensis,accounted for 4.88%.Nei’s gene diverdity,Shannon diversity index,coefficiednt of genetic differentiation(Gst)and fene flow were 0.0135,0.0215,1.0000 and 0.0000,respectively.The genetic identity and genetic distance of 17 samples were between 0.9634~1.0000 and 0.0000~0.0373,which indicate that there was very low level of genetic diverdity existed in Carpinus putoensis,and the relationship between parents and filial generation was very steadily.

【基金】 浙江省科技厅科技项目“国家重点保护野生植物保存技术研究”(001102203)
  • 【文献出处】 山东林业科技 ,Journal of Shandong Forestry Science and Technology , 编辑部邮箱 ,2011年01期
  • 【分类号】S792.99
  • 【被引频次】10
  • 【下载频次】198
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