【摘要】 本实验采用薄层色谱法对川楝子中香草酸、异香草酸进行定性鉴别;采用高效液相色谱法对川楝子中香草酸进行含量测定。色谱柱:Hypersil BDS C18(250 mm×4.60 mm,5μm),柱温:30℃,流动相:甲醇∶水∶冰乙酸=25∶75∶0.5,流速:1.0 mL.min-1,检测波长:260 nm。最终,定性鉴别斑点清晰。结果表明香草酸含量在1.022～16.352μg范围内,进样量与峰面积呈良好线性关系,相关系数r=0.9998;香草酸的平均回收率为102.45%,RSD=1.22%(n=6)。更多还原

【Abstract】 In this research,vanillic acid and iso-vanillic acid in Melia toosendan Sieb.were identified by TLC.The content of vanillic acid was determined by HPLC.The HPLC system consisted of BDS-C18,methanol-water-acetic acid(25∶75∶0.5) as mobile phase,flow rate of 1.0 mL/min and column tempreture of 30 ℃.The eluate was detected at 260 nm.The TLC spots developed were fairly clear.The control content of vanillic acid was within the range of 1.022～16.352 μg,and there was fine linearship between the sample input and the peak area.The average recovery was 102.45%,r=0.9998(n=6),RSD = 1.22%.更多还原