【摘要】 目的:探讨脂联素基因SNP+45(T/G)多态性与2型糖尿病(T2DM)氧化应激指标8-异前列腺素F2α之间的关系。方法:聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法检测120例T2DM患者(T2DM组)脂联素SNP+45(T/G)多态性,同时选取正常体检者60例为对照组,采用竞争酶免疫测定的方法检测T2DM患者血清8-异前列腺素F2α水平,检测各组空腹血糖、血脂及空腹胰岛素水平,计算HOMA-IR等指标。结果:T2DM患者血清8-异前列腺素F2α水平高于对照组,T2DM患者脂联素SNP+45位点GG基因型频率高于对照组(P<0.05)。T2DM组内GG型的体质量指数(BMI)高于TT及TG型(P<0.05);TT、TG及GG基因型血清8-异前列腺素F2α水平差异无统计学意义(P>0.05)。结论:脂联素SNP+45(T/G)多态性与T2DM氧化应激之间可能无相关关系。更多还原

【Abstract】 Objective:To investigate the relationship between adiponectin gene SNP +45(T/G) polymorphism and 8-isoprostaglandin F2α,a index of oxidative stress in type 2 diabetes(T2DM).Methods:The SNP +45(T/G) polymorphism was detected by PCR-RFLP in 120 T2DM patients.The serum concentration of 8-isoprostaglandin F2α was measured by enzyme-linked immunosorbent assay method.The control group?was selected from?healthy people.Results:The serum level of 8-isoprostaglandin F2α was higher in T2DM group than that of control group.The frequency of adiponectin SNP45 site of GG was higher in T2DM group than that of control group(P < 0.05).In T2DM group the value of body mass index(BMI) was higher in GG genotype compared with that of TT and TG genotypes(P < 0.05).There were no significant differences in the serum concentration of 8-isoprostaglandin F2α between TT,TG and GG genotypes(P > 0.05).Conclusion:There might be no significant relevance between adiponectin gene SNP +45(T/G) polymorphism and oxidative stress in type 2 diabetes.更多还原