【摘要】 以M-NH2-SiO2为载体,戊二醛为交联剂制备固定化漆酶,研究2,4-DCP(2,4-二氯苯酚)浓度、溶液酸度、温度对固定化漆酶降解2,4-DCP的影响.结果表明,固定化漆酶降解2,4-DCP最佳条件为:当2,4-DCP质量浓度为5mg.L-1,溶液pH为5.5,温度为30℃时,降解去除率为42.28%.固定化漆酶具有最大活性时的溶液pH值相比于自由酶的最佳pH向碱性偏移;与自由漆酶相比,固定化漆酶有良好的稳定性和重复使用性.更多还原

【Abstract】 Laccase was immobilized on M-NH2-SiO2 with glutaraldehyde as crosslinking agent.The effects on the activity of immobilized laccase,such as concentration of 2,4-DCP(2,4-dichlorophenols),acidity of solution,temperature were studied.The results show when concentration of 2,4-DCP is 5 mg·L-1,pH of solution is 5.5,and the temperature is at 30℃,the highest removal rate of 2,4-DCP is 42.28%.The results show that the optimal pH for immobilized laccase is higher than the best pH for free enzyme.Compared with the free enzyme,its stability and repetition are considerably improved.更多还原