【摘要】 目的了解临床分离的真菌分布,分析其药敏性,为临床感染性疾病提供病原学诊断和合理使用抗真菌药物的依据。方法收集2010年1-12月中南大学湘雅三医院住院患者送检标本,经血培养基或沙保罗培养基培养后,挑取酵母样菌落,进行菌种鉴定和药敏试验。结果分离到435株真菌,居前三位者分别为白色念珠菌(54.9%),近平滑念珠菌(20.5%),葡萄牙念珠菌(11.3%)。这些真菌主要来自重症监护室(ICU)(32.6%)和痰标本中(74.8%)。药敏结果显示,分离的酵母样真菌对5-氟胞嘧啶、两性霉素B和酮康唑敏感性较高,分别为97.5%、96.8%和95.8%。对氟康唑、伊曲康唑和益康唑耐药性较高,分别为47.4%、43.0%和21.2%。结论真菌临床分离菌株以白色念珠菌和近平滑念珠菌为主,临床真菌对5-氟胞嘧啶、两性霉素B和酮康唑具有较高的敏感性。更多还原

【Abstract】 Objective To investigate the distribution and drug sensitivity of fungi isolated from clinical specimens,and to provide the evidence for etiologic diagnosis and suitable antifungal management of clinical infective diseases. Methods Specimens from inpatients of the Third Xiangya Hospital of Central South University from January to December 2010 were collected and cultured on blood culture medium and Sabourand’s medium.After detecting by microscope,the yeast-like strains were selected and identified.Then the drug sensitivity tests of these strains were conducted. Results Among the selected 435 strains,the top three types of strains were Candida albicans(54.9%),Candida parepsilosis(20.5%) and Candida lusitaniae(11.3%).Most of the strains came from intensive care unit(32.6%) and sputum samples(74.8%).Susceptibility results showed that yeast-like fungi were highly sensitive to 5-fluorocytosine,amphotericin B and ketoconazole,with the sensitivity rates of 97.5%,96.8%,and 95.8% respectively,but highly resistant to fluconazole,itraconazol and econazole,with the resistance rates of 47.4%,43.0% and 21.2%,respectively. Conclusions Candida albicans and Candida parepsilosis strains were the main organisms in the clinical fungal infection.The clinical fungi were highly sensitive to 5-fluorocytosine,amphotericin B and ketoconazole.更多还原