【摘要】 目的探讨妊娠期接触苯对母鼠及其子鼠免疫功能的影响。方法 40只孕鼠被随机等分为空气对照组和5.0、10.0、15.0mg/m3三个不同浓度的苯染毒组,各组从孕7d开始,连续染毒至分娩,每天染毒2h。分别在分娩后的1d及7d,每组取5只母鼠和5只子鼠处死,取脾制备脾淋巴细胞;检测母鼠和子鼠脾淋巴细胞增殖力、细胞周期及细胞凋亡。结果在分娩后1d及7d,中、高浓度苯染毒组母鼠及子鼠的脾淋巴细胞增殖力均低于对照组(P<0.05),并有明显的剂量-效应关系(P<0.05);子鼠与母鼠的淋巴细胞增殖抑制存在较好的相关性。中、高浓度苯染毒组母鼠和子鼠脾脏G0/G1期淋巴细胞数高于对照组(P<0.05),S期淋巴细胞数低于对照组(P<0.05);各浓度苯染毒组母鼠、子鼠的脾脏淋巴细胞凋亡率高于对照组(P<0.05),并且存在剂量-效应关系。结论妊娠期鼠吸入苯可诱导母鼠及其子鼠脾脏淋巴细胞凋亡,淋巴细胞分裂被阻止在G0/G1期,并抑制脾脏淋巴细胞的增殖,使母鼠和子鼠免疫功能下降。更多还原

【Abstract】 Objective To explore the effect of benzene on proliferation and apoptosis of splenic lymphocytes in mother generation and offspring mice. Methods Forty pregnant mice were divided averagely into 4 groups at random.From the 7th day after pregnancy,each of group was exposed to benzene vapour until to parturition(0.0,5.0,10.0 and 15.0 mg/m3,respectively),2 hours per day. At the 1st and 7th days after parturition,5 mother generation mice and 5 offspring mice of each group were killed to detect the proliferation,cell cycle and apoptosis of splenic lymphocytes in mother generation and offspring mice by MTT assay and flow cytometric analysis. Results During the 1st day and 7th day after parturition,the proliferation of splenic lymphocytes of mother generation and offspring mice in the middle-and high-concentration of benzene groups was inhibited significantly in a concentration-dependent manner(P<0.05).The cell cycle of splenic lymphocytes of mother generation and offspring mice was blocked in G0/G1 phase in the middle-and high-concentration of benzene groups.The quantity of splenic lymphocytes apoptosis was increased significantly in a concentration-dependent manner in each benzene group(P<0.05). Conclusion Benzene exposure during pregnancy can damage the immunological function of mother generation mice and offspring mice.更多还原