【摘要】 目的探讨用丁二酸二异辛酯磺酸钠(aerosol OT,AOT)-异辛烷反胶束体系萃取番茄凝集素的可能性。方法考察并分析了反胶束溶液萃取番茄凝集素时的几种因素如pH值、表面活性剂AOT的浓度、KCl的浓度等对蛋白前萃率的影响。结果在KCl浓度为50 mmol·L-1、AOT浓度为110 mmol·L-1及pH值9.0条件下前萃取率为46.51%。结论以AOT-异辛烷反胶束溶液萃取番茄凝集素是可行的。更多还原

【Abstract】 Objective To explore the possibility of extraction of tomato lectin by aerosol OT(AOT)-isooctane reverse micelles system.Methods The effects of pH,AOT concentration,KCl concentration on the forward extraction rate were investigated and analyzed.Results At the condition of KCl concentration 50 mmol · L-1,AOT concentration 110 mmol · L-1 and pH 9.0,the forward protein extraction rate achieved 46.51%.Conclusions It is possible to extract tomato lectin using reversed micelles of AOT-isooctane.更多还原