【摘要】 目的探讨人胎盘源间充质干细胞(PMSCs)对单核源树突状细胞(mono-DCs)体外成熟的影响。方法采用密度梯度离心法分离人脐血单核细胞,在人粒细胞-巨噬细胞集落刺激因子(hGM-CSF)和人白细胞介素-4(hIL-4)刺激下获得大量未成熟DCs。将DCs和PMSCs共培养4 d,同时加入LPS刺激,分别通过倒置显微镜、流式细胞仪和混合淋巴反应(MLR)检测DCs的成熟。结果共培养DCs呈散在分布,细胞边缘圆滑;与成熟DCs相比,其表面CD80、CD86、CD83、CD40的表达明显较低,而CD14的表达较高;共培养组DCs刺激同种异型淋巴细胞增殖的能力在各个浓度均明显低于对照组(均P<0.05)。结论 PMSCs可以明显抑制脐血单核源DCs的体外成熟。更多还原

【Abstract】 Objective To study on the in vitro maturation of cord blood monocyte-derived dendritic cells(mono-DCs) regulated by human placenta-derived mesenchymal stem cells(PMSCs).Methods Human cord blood monocytes were isolated by density gradient centrifugation and cultured with human granulocyte-macrophage colony stimulating factor(hGM-CSF) and human interleukin-4(hIL-4).Then large amounts of immature DCs were obtained and co-cultured with PMSCs in the presence of LPS for 4 days.The effects of PMSCs on the maturation of DCs were analyzed with inverted microscope,flow cytometry and mixed lymphocyte reaction respectively.Results In co-culture group,DCs were scattering around and presented round and smooth round and under inverted microscope.Compared with the control group,co-cultured DCs expressed lower level of CD80,CD86,CD83 and CD40,but higher level of CD14.Allogeneic lymphocytes proliferation assay showed that the stimulatory abilities of DCs from co-culture group were lower than those in the control group(all P<0.05).Conclusion PMSCs can significantly suppress the maturation of cord blood mono-DCs in vitro.更多还原