【摘要】 目的研究烟曲霉对巨噬细胞吞噬能力及细胞活性的影响规律。方法通过免疫荧光法测定巨噬细胞吞噬烟曲霉后吞噬量的变化,同时利用MTT比色法测定巨噬细胞的细胞活性变化。结果在吞噬初期(20min),巨噬细胞吞噬乳胶颗粒的速度明显高于吞噬孢子(P<0.05),然而最终(70min)吞噬两种颗粒的能力无明显差异(P>0.05);巨噬细胞吞噬休眠孢子及热灭活孢子的量无显著差异(P>0.05);另外,休眠孢子、热灭活休眠孢子及膨胀孢子均可显著降低巨噬细胞的细胞活性(均P<0.05),而烟曲霉菌丝和乳胶颗粒对巨噬细胞的细胞活性无明显影响(P>0.05)。无色素的烟曲霉白化株休眠孢子不能使巨噬细胞活性降低(P>0.05)。结论烟曲霉孢子表面色素可能是降低巨噬细胞活性的影响因素。更多还原

【Abstract】 Objective To study the effect of different live-forms of Aspergillus fumigatus on both macrophage phagocytosis and cytoactivity.Methods Phagocytosis of Aspergillus fumigatus conidia and latex beads by macrophage was determined by GFP-expressing conidia and immunofluorescence;macrophage cytoactivity was measured by microplate-MTT colorimetric assay.Results At the early phase of phagocytosis(around first 20 min),latex beads were phagocytized more rapidly than conidia by macrophage (P<0.05),however,there was no significant difference in phagocytosis capacity between two of them(P>0.05).Moreover,heat-killing pretreatment on conidia did not affect its phagocytosis by macrophage (P>0.05 ).In addition,cytoactivity of macrophage was inhibited by the interaciton of macrophage with live rest conidia,swollen conidia and heat-killed conidia(P<0.05),but not hyphae and latex beads (P>0.05).Albino Aspergillus fumigatus conidia did not inhibited the cytoactivity of macrophage(P>0.05).Conclusion Melanin,which is present on the surfaces of Aspergillus fumigatus conidia,may be able to affect the cytoactivity of macrophage.更多还原