【摘要】 目的观察β-氨基丙腈(BAPN)对兔胆管良性狭窄形成的影响。方法通过钳夹法建立兔胆管狭窄模型,并给予BAPN腹腔内注射处理,半月后行胆管造影,测量胆管直径;行血清总胆红素和直接胆红素含量测定;取钳夹段胆总管组织进行病理学检查,测量胆管壁厚度、镜下观察病理改变及高倍镜下成纤维细胞单位面积密度计数。结果造影显示对照组钳夹部位见狭窄环,以上胆管明显扩张,实验组胆管扩张不明显。实验组血清总胆红素和直接胆红素无明显升高。病理示对照组成纤维细胞增生活跃,胆管壁显著纤维化增厚,炎性细胞广泛浸润,VanGieson染色见胆管黏膜下大量胶原纤维增生,排列致密紊乱,实验组胆管壁增厚不明显,成纤维细胞增生少,VanGieson染色见胆管黏膜下胶原纤维增生不明显,排列较为整齐。结论良性胆管狭窄主要病理变化是胆管壁成纤维细胞增殖旺盛并胶原纤维过度增生;BAPN可以抑制胆管成纤维细胞的过度增殖和胶原纤维的交联,对胆管良性狭窄起到一定预防作用。更多还原

【Abstract】 Objective To investigate the effects of β-Aminopropionitrile on benign biliary stricture in rabbit.Methods The benign biliary stricture model was established by clamping extrohepatic bile duct in rabbit,then intraperitoneal perfusion daily the experimental group with the BAPN(500 mg/kg),while the blank control group and control group with normal saline.Half a month later,all animals were performed to make cholangiography and measure the diameter of bile duct 0.5 cm above the clamped part,collect blood samples from the inferior vena cava to determine the serum levels of TBIL and DBIL,the samples of the common bile duct were extracted for microscopic examination to observe the morphological changes,measure the bile duct wall thickness,and count the fibroblasts density of per unit area under high-power microscope.Results In the control group,narrow ring of the bile duct which was clamped partly was obviously seen in cholangiography,and the bile duct above the clamped site was dilated more significant than those in the experimental group.The serum levels of TBIL and DBIL were significant higher in the control group than those in the experimental group and the blank control group.Microscopic examination showed,in the control group,fibroblast proliferation of clamped part of the bile duct was actively,the bile duct wall was thicken for significant tissue fibrosis,inflammatory cells were extensive infiltrative,and a large number of bile duct hyperplasia of collagen fibers with spreading densely and disorder were seen in submucosal by VanGieson staining,while all of those in the experimental group were not remarkable.Conclusion It was proved that there were exuberant proliferation of fibroblasts and excessive synthesis of collagen fibers in benign stricture of bile duct.BAPN can play a role in the prevention and treatment of bile duct stricture through inhibition of the excessive fibroblast proliferation and collagen fiber crosslinking.更多还原