【摘要】 从水体中分离能够降解亚硝酸盐的乳酸菌。参照《伯杰细菌鉴定手册》对分离出的wyq-1、wyq-2和wyq-3株菌进行生理生化特性鉴定,并利用分子生物学方法进行16SrRNA序列分析,参照国家标准方法GB/T 5009.33-2008中的格里斯比色法测定降解亚硝酸盐的能力。结果表明:3株菌均为乳杆菌属(Lactobacillus),wyq-1为发酵乳杆菌(Lactobacillus fermentum)、wyq-2为嗜酸乳杆菌(Lactobacillus acidophilus)、wyq-3为植物乳杆菌(Lactobacillus plantarum)。对3株菌在添加血红素的MRS液体培养基中和猪肉培养基中降解亚硝酸盐的能力进行了测试,菌株wyq-3降解能力最好,降解率分别为87.0%和69.4%。研究结果表明用乳酸菌降解肉制品中的亚硝酸盐可以取得较好的效果。更多还原

【Abstract】 Isolated Lactobacillus from water was used to degrade nitrite.The strains wyq-1,wyq-2 and wyq-3 were identified by the characteristics of morphology,physiology and biochemistrytests and 16S rRNA sequence was analysed by molecular biological method.The degradation nitritea ability was based on GB/T 5009.33-2008.The three strains were identified as Lactobacillus fermentum,Lactobacillus acidophilus and Lactobacillus plantarum.The degradation nitritea ability was detected in the hemoglobin MRS liquid medium and pork medium,the degradation rate of wyq-3 was 87.0% and 69.4%.Nitritea in meat products can be degradated well by Lactobacillus.更多还原