【摘要】 目的利用从临床乳腺炎奶牛乳样中分离纯化的金黄色葡萄球菌(S.aureus)感染小鼠乳腺,建立奶牛金黄色葡萄球菌乳腺炎小鼠模型。方法将产后8～10 d的ICR远交系母鼠随机分为3组,包括无菌生理盐水对照组,低剂量攻菌组(含金黄色葡萄球菌2×102CFU)和高剂量攻菌组(含金黄色葡萄球菌5×106CFU)。以小鼠第4对乳腺为攻菌部位,用非损伤方法经乳头管注入生理盐水或金黄色葡萄球菌。攻菌24h后采集乳汁进行白细胞计数;从眼球采血,进行血常规检测和流式细胞计数;之后处死母鼠,取乳腺组织进行匀浆细菌计数,并做乳腺组织切片。结果高剂量攻菌组不论是体温、组织学观察,还是乳汁白细胞计数、乳腺匀浆细菌计数、血常规指标和流式细胞计数,都和对照组有显著或极显著差异(P<0.05);而低剂量攻菌组与对照组相比,除血液CD4+/CD8+比值差异显著外(P<0.05),其余指标差异均不显著。结论金黄色葡萄球菌浓度为5×106CFU、攻菌时间24 h时,成功建立了远交系小鼠金黄色葡萄球菌乳腺炎模型,而2×102CFU细菌浓度则不适于远交系小鼠乳腺炎模型的建立。更多还原

【Abstract】 Objective Infect mouse mammary gland with Staphylococcus aureus(S.aureus) isolated and purified from clinical mastitis milk samples in order to establish a mouse model of Staphylococcus aureus of dairy cows mastitis.Methods 8~10 days postpartum far inbred ICR female mice were randomly divided into three groups,including the sterile saline control group,low-dose-bacteria-attack group(2×102 CFU of Staphylococcus aureus) and high-dose-bacterial-attack group(5×106CFU of Staphylococcus aureus).Staphylococcus aureus or sterile saline were injected into the fourth pair mammary gland through the nipple tube with non-invasive method.24 h after bacteria attack,milk of these rats was collected to do white blood cell count,and blood from eyes to do blood testing and flow cytometry;after the female mice were killed,taking the breast tissue to do homogenate bacterial count,and do biopsy.Results The high-dose group have a significant or very significant difference(P<0.05) with the control group whether in the histological observation and body temperature,or milk white blood cell count,breast homogenate count,some indicators in blood testing and flow cytometry.But compared the low-dose group with the control group,indicators have no significant differences except the blood CD4+/CD8+ ratio was significantly different(P<0.05).Conclusion The distant strains of mice models of S.aureus mastitis was successfully established when the concentration of Staphylococcus aureus is 5×106CFU,bacterial attack for 24 h,and concentration of 2×102 CFU of bacteria is not suitable for the establishment of distant strains of mice models of S.aureus mastitis.更多还原