【摘要】 目的探讨缺氧诱导因子-1α（HIF-1α）基因转染是否提高骨髓间充质干细胞（MSC）缺氧状态下的葡萄糖摄取能力以及这种能力是否与葡萄糖转运载体-4（GLUT-4）的表达和易位有关。方法将MSCs分为常氧非转染组（即对照组）、常氧转染组、缺氧非转染组和缺氧转染组,分别于常氧（5%CO₂,37℃）和缺氧（94%N₂、1%O₂和5%CO₂,37℃）条件下孵育8 h。放射同位素法检测³H-G摄取量,免疫细胞化学和Western-blot检测GLUT-4的表达。结果①与缺氧非转染组相比,缺氧转染组显著提高细胞摄取³H-G量（P<0.01）,但二者均显著低于对照组和常氧转染组对³H-G的摄取（P<0.01）;②与缺氧非转染组相比,缺氧转染组显著提高细胞和细胞膜GLUT-4蛋白的表达（P<0.01）,2组均显著低于对照组GLUT-4蛋白的表达和移位（P<0.01）;③³H-G摄取量与细胞膜中GLUT-4表达呈正相关（r=0.437,P=0.001）。结论 HIF-1α基因提高MSCs的葡萄糖摄取能力,此机制与HIF-1α基因提高GLUT-4的表达和易位相关。更多还原

【Abstract】 Objective To elucidate whether hypoxia induced factor-1α（HIF-1α） gene improves the capability of glucose intake in hypoxia bone mesenchymal stem cells（MSCs）,and whether the transaction and the expression of GLUT-4 play an important role in this process ex vivo.Methods MSCs were randomly divided into control （normoxia non-HIF-1αtransfection）,normoxia HIF-1αtransfection group,hypoxia non-HIF-1αtransfection group,hypoxia Hif-1αtransfection group,and then each group was cultured with normoxia（5%CO₂ at 37℃） or hypoxia（94%N₂,1%O₂,5%CO₂ at 37℃） for 8 h.Finally,glucose uptake and the expression of GLUT-4 in cells and in cell membrane were assayed.Results①³H-G intake of MSCs was significantly increased in HIF-1αgene transfection than in non-HIF-1αgene transfection（P<0.01） when hypoxia,however,those were significantly lower in each hypoxia group than in each normoxia group（P<0.01）;②Under hypoxia,HIF-1αsignificantly increased the expression of GLUT-4 protein in cells and in cell membranes（P<0.01）,which were significantly decreased than in control group and in normoxia HIF-1αtransfection group（P<0.01）;③There was significant positive relation between ³H-G intake and GLUT-4 protein in plasma membrane（r = 0.437,P = 0.001）. Conclusion HIF-1αgene significantly improved the capability of glucose intake of hypoxia MSCs,which was mediated by the expression and transaction of GLUT-4.更多还原