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山西省甘蓝黄叶病病原菌的分离与鉴定
Isolation and Identification of the Pathogens Causing Cabbage Fusarium wilt in Shanxi Province
【摘要】 近年来山西省甘蓝黄叶病发生严重,造成了严重的经济损失。本试验采用常规组织分离法对山西省寿阳县甘蓝黄叶病的病原菌进行了分离、纯化,从采集的12份罹病植株根、茎部共分离到28个菌株,经形态学鉴定,分别为尖孢镰刀菌(Fusarium oxysporum)、木贼镰刀菌(F.equiseti)与砖红镰刀菌(F.lateritium),致病性测定结果表明,尖孢镰刀菌与木贼镰刀菌为山西省甘蓝黄叶病的病原菌,木贼镰刀菌致病性较尖孢镰刀菌弱。此外,木贼镰刀菌作为甘蓝黄叶病病原为首次报道。这一结果为进一步研究和控制该病害提供了可靠的理论依据。
【Abstract】 Cabbage in Shanxi province suffered serious losses from wilt disease in resent years.The possible pathogens of cabbage Fusarium wilt were isolated and purified from the diseased samples of cabbage in Shouyang,Shanxi by the conventional tissue method.The total 28 isolates were isolated from the roots and stems of 12 collected cabbage samples.Based on the morphological identification,which were identified as Fusarium oxysporum,F.equiseti and F.lateritium,respectively.The results of pathogenicity experiment showed that F.oxysporum and F.equiseti were the pathogens of cabbage Fusarium wilt,and F.oxysporum had a stronger pathogenicity than F.equiseti.F.equiseti was first reported as the pathogen of cabbage wilt disease.These results provided a basis for further study and control practice of the disease.
【Key words】 Cabbage Fusarium wilt; Pathogens; Isolation and purification; Morphological identification; Pathogenicity experiment;
- 【文献出处】 山西农业大学学报(自然科学版) ,Journal of Shanxi Agricultural University(Natural Science Edition) , 编辑部邮箱 ,2011年06期
- 【分类号】S436.35
- 【被引频次】6
- 【下载频次】128