【摘要】 研究汉黄芩素对人神经胶质瘤细胞U251增殖和凋亡的影响,用不同浓度的汉黄芩素作用于U251细胞系,通过核染色观察形态变化,MTT检测细胞增殖抑制率,流式分析检测细胞的凋亡情况.结果显示,药物作用后的细胞,DAPI核染色呈致密浓染,并伴有凋亡小体的出现;MTT法测得U251细胞的增殖得到明显的抑制,抑制率随浓度和作用时间的增加而上升,其中作用24h的IC50为145.87μmol/L;Annexin V-FITC/PI流式细胞检测证实汉黄芩素确实可以诱导U251细胞凋亡,并有剂量依赖的性质;通过比色法证明在药物处理过的细胞中,凋亡标志性酶Caspase-3被激活,且活性随着药物浓度的升高而增强.研究表明,汉黄芩素能明显抑制U251细胞增殖,诱导细胞凋亡,具有抗神经胶质瘤的作用.更多还原

【Abstract】 To investigate the effect of wogonin on the proliferation and cell apoptosis of the human glioma cell line U251,the cultured cells was treated with the different concentrations and times of wogonin,and the morphology of U251 nuclear atypia was observed after DAPI nuclear fluorescence staining.The cell proliferation inhibition rate was detected by MTT assay.The cell apoptosis was detected by Annexin V-FITC /PI straining and flow cytometry.DAPI staining showed partial cells presented morphological changes of apoptosis;MTT assay showed the cells’ proliferation rate was obviously inhibited in a dose and time-dependent manner,and the IC50 for 24 h wogonin treated U251 cells was 145.87 μmol/L;flow cytometry showed that U251 cells’ apoptosis rate increased significantly in a dose dependent manner.Also the cells showed Caspase-3 activity and the activity was increased as the concentration of wogonin was increased.It suggested that wogonin can inhibit the proliferation of U251 cells and induce apoptosis.It has the potential effect of anti-gliomas.更多还原