Loop-mediated isothermal amplification(LAMP) is a novel technique for genetic diagnosis,which was published on Nucleic Acids Res in 2000 by Notomi,a Japanese scientist.It is a simple,efficient and sensitive DNA amplification method which has been widely used in various fields including food,clinic and agriculture,and might be a mainstream method for genetic diagnosis.This paper reviews the reaction characters,principle,advantage,disadvantage as well as clinical application of LAMP.
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