【摘要】 目的检测髓母细胞瘤中PI3K、AKT及Nrf2蛋白的表达,探讨PI3K/AKT信号传导通路在髓母细胞瘤中的作用机制及其临床意义。方法应用免疫组化Envision二步法检测33例髓母细胞瘤及17例对照脑组织中PI3K、AKT及Nrf2蛋白的表达,分析3种蛋白之间表达的相关性及其与髓母细胞瘤临床病理特征及类型的相关性。结果在33例髓母细胞瘤中,PI3K、AKT及Nrf2蛋白的表达率显著高于对照脑组织(P<0.05);PI3K、AKT及Nrf2蛋白的表达显著相关(P<0.05),而与患者年龄、性别、肿瘤大小及肿瘤类型均无显著相关性(P>0.05)。结论 PI3K/AKT信号传导通路可能对髓母细胞瘤的发生、发展起重要作用,可能成为髓母细胞瘤诊治的新靶点。更多还原

【Abstract】 Objective To determine the expressions of PI3K,AKT and Nrf2 in medulloblastoma,and investigate the action mechanism of PI3K / AKT signal pathway in medulloblastoma as well as its significance in clinic.Methods The expressions of PI3K,AKT and Nrf2 in 33 medulloblastoma specimens and 17 control brain tissue specimens were determined by immunohistochemical assay with two-step Envision staining,based on which the relationship between expressions of the three kinds of proteins as well as their relationship to clinical pathological character and type of medulloblastoma.Results The expression rates of PI3K,AKT and Nrf2 in 33 medulloblastoma specimens were significantly higher than those in control brain tissue specimens(P < 0.05).Significant relationship was observed between the expressions of the three kinds of proteins(P < 0.05).However,the expressions showed no significant relationship to the age,sex or tumor size and type(P > 0.05).Conclusion PI3K / AKT signal pathway might play an important role in onset and progress of medulloblastoma and be used as a new target for diagnosis and therapy of medulloblastoma.更多还原