【摘要】 目的探讨IL-27p28基因启动子区g.-964 T>C、第2外显子区g.2905T>G和第4外显子区g.4730T>C 3个位点的多态性与中国朝鲜族人群哮喘的相关性。方法选取32例中国朝鲜族哮喘患者(病例组)和93名中国朝鲜族正常人(对照组)作为观察对象,采用PCR法扩增IL-27p28基因g.-964 T>C、g.2905T>G和g.4730T>C位点基因片段,单碱基延伸法进行基因分型,分析病例组和对照组的基因型频率及等位基因频率。结果病例组和对照组的基因型分布均符合Hardy-weinberg平衡定律(P>0.05)。g.-964位点的基因型频率及等位基因频率在病例组和对照组之间的分布差异有统计学意义(P<0.001);g.2905和g.4730位点的基因频率及等位基因频率在病例组和对照组之间的分布差异无统计学意义(P>0.05)。结论 IL-27p28基因多态位点g.-964 T>C可能与朝鲜族人群哮喘易感性相关,g.2905T>G和g.4730T>C多态性与朝鲜族人群哮喘未发现有相关性。更多还原

【Abstract】 Objective To investigate the relationship of polymorphisms at g.-964T > C site in promoter region,g.2905T > G site in the second exon region and g.4730T > C site in the fourth exon region of IL-27p28 gene to the asthma in Chinese Korean Nationality population.Methods The g.-964 T > C,g.2905T > G and g.4730T > C sites of IL-27p28 gene were amplified from the blood of 32 Chinese Korean Nationality patients with asthma by PCR for genotyping by single nucleotide extension method,and analyzed for genotype and allele frequencies,using those from 93 healthy persons of the same nationality as control.Results Both the distributions of genotypes in trial and control groups were fitted to Hardy-weinberg Law(P > 0.05).However,the genotype and allele frequencies at g.-964T > C site in trial group showed significant difference(P < 0.001),while those at g.2905 and g.4730 sites showed no significant difference with those in control group(P > 0.05).Conclusion The polymorphism at g.-964T > C site of IL-27p28 gene might be related to the susceptibility to asthma in Chinese Korean Nationality population.However,no relationship was observed between the polymorphism at g.2905T > G or g.4730T > C sites and asthma in the said population.更多还原