【摘要】 目的探讨激活素A(Activin A)及激活素结合蛋白(Follistatin,FS)在急性酒精性肝损伤小鼠肝组织中的表达。方法通过1次/12 h给予小鼠5 g/Kg酒精连续灌胃3次,复制急性酒精性肝损伤小鼠模型,采用实时定量RT-PCR检测小鼠肝组织Activin A及FS mRNA表达水平,免疫组织化学染色观察小鼠肝组织Activin A及FS蛋白的表达水平。结果急性酒精性肝损伤小鼠肝组织Activin A mRNA和蛋白表达水平均显著高于对照组(P<0.01);FS mRNA和蛋白表达水平与对照组比较,差异均无统计学意义(P>0.05)。结论急性酒精性肝损伤小鼠肝组织Activin A-FS表达失衡,以Activin A升高为主,提示Activin A-FS系统失衡可能与酒精性肝损伤有关。更多还原

【Abstract】 Objective To investigate the expression of activin A and activin-binding protein(Follistatin,FS) in liver tissues of mice with acute alcohol-induced liver lesion.Methods Mice were administered with alcohol by gavage at a dosage of 5 g / kg,once 12 h for 3 times,to copy the mouse model of acute alcohol-induced liver lesion.The transcription levels of activin A and FS mRNAs in liver tissues of mice were determined by real-time quantitative RT-PCR,while the expression levels of activin A and FS by immunohistochemical staining.Results Compared with those in control group,the mRNA and protein levels of activin A in liver tissues of model mice were significantly high(P < 0.01),while those of FS showed significant difference(P > 0.05).Conclusion The expression of activin A-FS in liver tissues of mice with acute alcohol-induced liver lesion was unbalanced,which was mainly expressed as increased activin A,indicating that the unbalance of activin A-FS system might be associated with alcohol-induced liver lesion.更多还原