【摘要】 目的探讨自体血小板胶(Autologous platelet-rich gel,APG)中转化生长因子-β1(Transforming growth factor beta1,TGF-β1)浓度的影响因素。方法用一体性塑料血袋分别采集40名健康人的静脉血50 ml,采用两次离心法制备富血小板血浆(Platelet-rich plasma,PRP),用血细胞分析仪检测静脉血和PRP中的血小板数目;将PRP与凝血酶和钙剂混合液按体积比10∶1的比例混合,制备APG,采用ELISA法定量分析APG中的TGF-β1浓度;选择5个参数(年龄、性别、静脉血血小板计数、PRP中血小板平均计数及静脉血制备前保存温度)进行多元线性回归分析。结果 PRP中血小板平均计数[(1 232.23±195.45)×109个/L]约为静脉血[(195.95±42.29)×109个/L]的6倍;APG中TGF-β1的平均浓度为(5.83±2.31)ng/ml,与制备前静脉血保存温度密切相关(P<0.000 5),而与年龄、性别、静脉血血小板计数和PRP中血小板计数无关。结论制备前APG的静脉血样本应保存于室温(20～24℃)下。更多还原

【Abstract】 Objective To investigate the influencing factors of transforming growth factor β1(TGF-β1) concentration in autologous platelet-rich gel(APG).Methods Venous blood were collected from 40 healthy volunteers,50 ml for each,by integrated plastic blood collection bags,with which platelet-rich plasma(PRP) was prepared by two steps of centrifugation.The platelets in venous blood and PRP were counted by blood cell analyzer.APG was prepared by mixing PRP with the mixture of thrombin and calcium,at a volume ratio of 10 ∶ 1 and determined for TGF-β1 concentration by ELISA.Multiple linear regression analysis was performed on five parameters,i.e.age,sex,platelet count in venous blood,mean platelet count in PRP and temperature for storage of venous blood before preparation of PRP.Results The mean platelet count in PRP[(1 232.23 ± 195.45) × 109 platelets / L] was about 6 times higher than that in venous blood [(195.95 ± 42.29) × 109 platelets / L].The mean concentration of TGF-β1 in APG was(5.83 ± 2.31) ng / ml,which was closely related to the temperature for storage of venous blood before preparation of PRP while was unrelated to age,sex as well as platelet counts in venous blood and in PRP.Conclusion The venous blood before preparation of PRP should be stored at room temperature(20 ～ 24℃).更多还原