【摘要】 pi-hit-1基因是本实验室通过空间诱变找到的一个水稻新基因。为了对pi-hit-1基因启动子结构和功能进行研究,首先使用植物启动子分析数据库(PlantProm DB-TSSP,TFSEARCH,PLACE及PlantCARE)对该基因转录调控区序列进行预测分析,结果显示该基因上游调控区存在多个顺式元件,主要集中在翻译起始位点前300bp的区域,转录起始位点位于翻译起始位点前100bp,在转录起始位点前132bp存在TATA box元件。凝胶电泳迁移率实验(EMSA)发现翻译起始位点上游约300bp存在转录因子特异结合位点,为该基因的核心启动子,这与预测结果一致。采用系统生物学的方法研究水稻新基因pi-hit-1启动子结构,发现了该基因的核心启动子元件,为研究空间环境如何影响基因的转录调控提供了重要依据。更多还原

【Abstract】 pi-hit-1 gene was a novel gene identified from space flight rice,and was predicted as a blast related gene.To analyze the structure and function of pi-hit-1 promoter,the gene promoter analyze softwares(PlantProm DB-TSSP,TFSEARCH,PLACE & PlantCARE)were used to predict the promoter of pi-hit-1 gene.Result showed there are some cis-elements in about 300bp upstream of pi-hit-1 gene.The transcription start site is at 100 bp upstream of the translation start site.There is a TATA box in 132bp upstream of the transcription stare site.EMSA was used to analyze the promoter.The result showed that the sequence of the sample was same to the predict sequence about the TATA box.更多还原