【摘要】 目的:介绍了一种从普通琼脂糖电泳中回收DNA的简便、快捷、高效且廉价的方法。方法:利用0.5 mL离心管、1.5mL离心管、尼龙膜做成的一个小装置。把含有DNA的凝胶放在膜上,离心,收集从管底流出来的液体,用乙醇沉淀DNA。结果:最终回收率为60%左右,回收率大约为市售试剂盒的90%,接近市售DNA回收试剂盒。结论:该方法操作简单,回收率高,无其他试剂污染。更多还原

【Abstract】 Objective: Introduced one of easy,rapid,effective and cheap methods for recovering DNA from agarose gel.Method: A recovering unit was made by using 0.5 mL microcentrifuge tube,1.5 mL microcentrifuge tube,nylon membrane.Put the agarose gel slices on the nylon membrane,then centrifuging after freezing the gels,and using alcohol to precipitate the DNA.Result: The recovery rate of this method was about 60%.Recovery is approximately 80% of commercially available kits.Conclusion: This method can efficient recover DNA from agarose gel without any possible contamination.更多还原