【摘要】 从扬子石化的废水淤泥中筛选到1株能发酵液体石蜡产脂肽类生物表面活性剂的假丝酵母CandidaE-2。通过单因子实验和正交试验,得到了最佳发酵培养基组成(g/L):牛肉膏3.0,蔗糖2.0,酵母膏0.25,KH2PO412.5,MgSO40.3,NaCl 1.5,CaCl20.05,尿素0.5;液体石蜡10%(体积分数)。最佳培养条件:初始pH7.0,接种量0.12 g/L,装液量为200mL三角瓶30 mL,培养时间为5 d。最终产量提高了2.7倍,达1.582 g/L。更多还原

【Abstract】 Candida E-2,a lipopetide biosurfactant producing strain,was isolated from sludge of wastewater treatment factory of Yangzi Petroleum Co.Ltd.The optimized medium composition by the single-factor experiment and orthogonal experiment was obtained(g/L): beef extract 3.0,sucrose 2.0,yeast extract 0.25,KH2PO4 12.5,MgSO4 0.3,NaCl 1.5,CaCl2 0.05,urea 0.5,initial pH 7.0,and liquid paraffin 10%(V/V).The optimized medium condition were inoculum amount 0.12 g/L,fermentation volume 30 mL/200 mL,fermentation time 5 d.Under such conditions the biosurfactant yield could rise 2.7 times and reach 1.582 g/L.更多还原