【摘要】 通过研究马来酸酐改性聚乳酸(MPLA)和聚乳酸(PDLLA)材料表面对MC3T3-E1成骨细胞形态、黏附、增殖、细胞总蛋白含量、碱性磷酸酶活性及细胞分泌无机钙含量的影响,评价MPLA和PDLLA材料的细胞相容性。结果显示:与PDLLA相比,MPLA材料上的成骨细胞完全黏附和充分铺展;MPLA材料上细胞的增殖速率、细胞总蛋白含量、细胞碱性磷酸酶活性及细胞分泌的无机钙含量都显著高于PDLLA(P<0.01)。这些结果说明,MPLA材料能促进MC3T3-E1成骨细胞的黏附、铺展、增殖及蛋白质的合成,并能促进成骨细胞的分化和矿化,与PDLLA材料相比具有更好的细胞相容性。更多还原

【Abstract】 The main objective of this study was to observe the adhesion,proliferation and differentiation of mouse osteblast-like MC3T3-E1 cells cultured on maleic anhydride-modified poly(D,L-lactic acid)(MPLA) and poly(D,L-lactic acid)(PDLLA) polymers,and to evaluate the cytocompatibility of MPLA polymer.The effects of MPLA and PDLLA polymers on the morphology,adhesion,proliferation,the content of total cellular protein,alkaline phosphatase(ALP) activity and the content of Ca of MC3T3-E1 cells were explored.These results indicated that MC3T3E1 cells on MPLA polymer adhered and spread more fully.On MPLA polymer,the proliferation,total protein content,ALP activity,Ca content of the cells were significantly higher than those of the cells on PDLLA polymer(P<0.01).It was concluded that MPLA polymer could promote the adhesion,spreading,proliferation and the synthesis of protein of osteoblasts,and also induced the differentiation and mineralization of osteoblasts,suggesting that MPLA polymer might have the better cytocompatibility than PDLLA.更多还原