【摘要】 联合应用机械法和去垢剂-酶消化法制备小肠黏膜下层(SIS),并以其作为支架材料,将体外培养扩增的软骨细胞接种于支架,体外构建组织工程软骨。体外培养3周,观察软骨细胞在支架材料上的生长分布情况。结果显示软骨细胞在制备的SIS上可较好地黏附、增殖,并且分泌大量Ⅱ型胶原和GAG;但软骨细胞不能长入到SIS内部,只能在其表层和表面的结构中生长。结论认为SIS支架材料均具有良好的细胞相容性和表面活性,并且促进软骨细胞增殖和维持软骨细胞表型。但需要进一步了解SIS结构和成分对软骨细胞游走趋化能力的影响,促进软骨细胞均匀分布在材料上。更多还原

【Abstract】 This paper is aimed to investigate the feasibility of applying the small intestine submucosa(SIS) as the scaffold in constructing tissue engineering cartilage in vitro.We obtained SIS from the small intestine of specific pathogen-free pigs.Then we isolated tunica submucosa layer from the mucosal,muscular, and serosal layers by gentle mechanic abrasion.The SIS was made acellular by combination of detergent and enzyme digestion.The chondrocytes were seeded onto the SIS and were cultured for 3 weeks.The cell growth,attachment and distribution were detected by histochemical stain,immunohistochemical stain and scan electron microscope.The chondrocytes could adhere and grow well on the matrix surface,and synthesize a large of the GAG and typeⅡcollagen.However,the chondrocytes grew only on the surface and superficial layer of the scaffold,they did not move into the inner part of the scaffold.It could be concluded that SIS has good cellular compatibility without cytotoxicity and provides temporary substrate to which these anchorage-dependent cells can adhere,and stimulate the chondrocytes anchored on the scaffold to proliferate and keep differentiated phenotype.Further study will be needed to promote the ability of chondrocyte chemotaxis in order to distribute the chondrocytes into the whole scaffold uniformly.更多还原