【摘要】 目的:探讨胎盘Hofbauer细胞在乙型肝炎病毒(HBV)母婴垂直传播的作用。方法:垂直传播组(母亲及其新生儿血HBsAg和HBV-DNA均为阳性)、非垂直传播组(母亲血HBsAg和HBV-DNA阳性而新生儿阴性)和对照组(母亲及其新生儿血HBsAg和HBV-DNA均为阴性)各30例,透射电了显微镜观察其胎盘Hofbauer细胞超微结构变化及其与HBV颗粒的关系。结果:①对照组未发现Hofbauer细胞:在垂直传播组和非垂直传播组,Hofbauer细胞散在分布于胎盘间质中。②在垂直感染组,Hofba-uer细胞肿胀,胞浆突起减少,胞浆空泡变大;粗面内质网及高尔基复合体萎缩,线粒体缩小,溶酶体少见;胞核增大,染色质浓缩、边聚。在非垂直感染组,Hofbauer细胞呈圆形或卵圆形,细胞表面有大量胞浆突起,排列着微吞饮小体,细胞胞浆内有圆形空泡;线粒体呈杆状,峭排列紧密,溶酶体较多,高尔基体及粗面内质网欠发达;胞核偏位,核仁显著,染色质分布均匀。③在Hofbauer细胞胞质空泡、细胞间隙内存在单个或多个成熟病毒颗粒、病毒包涵体和病毒抗原颗粒。结论:HBV可引起胎盘Hofbauer细胞超微结构改变,并经Hofbauer细胞介导引起母婴垂直传播。更多还原

【Abstract】 Objective: To investigate the role of Placental Hofbauer cells In Mother-to-neonate vertical transmission of hepatitis B virus(HBV).Methods: In vertical transmission cases,non-vertical transmission cases and controls(n=30 respectively),ultrastr-ucture changes of Hofbauer cells and HBV situation in placenta were observed by transmission electronic microscopy.Results:①Hofbauer cells were not found In Control,but widely distributed in placental Intercellular space In the vertical and non-vertical transmi-ssion cases.②In the vertical transmission cases,Hofbauer cells swelling,cell vacuolization;rough endoplasmic reticulum hypertro phy,mitochondrion construction;trophocyte chromating collecting to side.In the non-vertical transmission cases,Hofbauer cells③Single or more viral particle,viral inclution body,viral surface antigen particle appeared In cytoplasm cavity of Hotbauer cell.Conclusion: HBV can result In ultramicrostructure changes of plcental Holbauer cells,and can be transmitted into the babies via Hofbauer cells.更多还原