【摘要】 目的:明确鳞癌感染人类疱疹病毒8型(HV-8)ORF75基因亚型分类并初步探讨其与鳞癌的相关性。方法:对40例皮肤鳞癌、46例食管鳞癌石蜡包埋组织进行HHV-8 DNA抽提、扩增、双向测序,使用DNASTAR软件、Crustal W软件和PHYLIP软件包对测序结果进行系统发生学分析,从而确定HHV-8 ORF75基因亚型,最后运用Fisher确切概率法对结果进行统计学分析。结果:①40例皮肤鳞癌有10例HHV-8感染为阳性,阳性率为25.0%;46例食管鳞癌有12例为阳性,阳性率为26.1%.②皮肤鳞癌感染HHV-8 ORF75亚型6例为A亚型,4例为C亚型;食管鳞癌感染HHV-8 ORF75亚型8例为A亚型,4例为C亚型③鳞癌感染HHV-8 ORF75基因亚型与鳞癌患者感染部位及病理分级之间没有统计学意义(P>0.05)。结论:鳞癌患者感染HHV-8 ORF75亚型属于A亚型和C亚型;鳞癌感染HHV-8 ORF75基因亚型与鳞癌患者感染部位及病理分级无关。更多还原

【Abstract】 Objective: To study the distributional feature of ORF26 subtypes of Human herpesvirus 8(hhv-8) in Xinjiang Squalors cell carcinoma(SCC) cases.Methods: HHV-8 DNA were amplified by nested-PCR and sequenced which was extracted from paraffin-em-bedded tissue of 41 skin Squalors cell carcinoma cases and 46 esophagus Squalors cell carcinoma.The phylogenetic analysis were car-ried out by using the software DNASTAR,the program Crustal W and the PHYLIP package.The data were analyzed by Spss 17.0.Results: ①HHV-8 DNA was detected in 9(21.95%) of 41 skin SCC cases and 10(21.74%) of 46 Esophagus SCC cases.②7 of 9 HHV-8 positive cases were ORF26 A subtype,2 were C subtype in skin SCC cases.A and C subtype in Esophagus SCC cases were 7 and 3 respectively.Conclusion: The subtype of ORF26 in Xinjiang SCC cases who infected HHV-8 involved A and C subtype.A subtype was more than C subtype.The different ORF26 subtypes of HHV-8 have nothing to do with gender,age,race and position of SCC cases.更多还原