【摘要】 目的:研究黑龙江地区汉族人2型糖尿病家系的LEPR基因Gln223Arg多态性,探讨其与2型糖尿病发病的关系。方法:应用聚合酶链式反应-限制性内切酶长度多态性(PCR-RFLP)技术,对来自于黑龙江地区120个2型糖尿病家系中的210例2型糖尿病患者及319例正常对照的LEPR基因Gln223Arg(668 A→G)位点进行基因分型。结果:LEPR基因Gln223Arg三种基因型在病例组和对照组间整体分布有统计学意义(P=0.034,df=2);除AG基因型(x2=4.550,P<0.01)外,其余各基因型及等位基因在病例组和对照组间分布未见显著性差异(P>0.05)。结论:LEPR基因Gln223Arg多态性与黑龙江地区汉族人2型糖尿病有关,LEPR基因可能为中国人2型糖尿病发病的相关易感基因。更多还原

【Abstract】 Objective: To detect LEPR gene Gln223Arg polymorphism in Heilongjiang Han families with type 2 diabetes and to investigate their relationship with type 2 diabetes.Methods: PCR-RFLP method was used to test the polymorphisms of LEPR Gln223Arg(668 A→G) in 210 individuals from 120 type 2 diabetes families and 319 normal control subjects in Heilongjiang area.Results: The overall distribution of the three genotypes of LEPR gene Gln223Arg between cases and controls was significantly different(P=0.034,df=2).Except AG genotype(x2=4.550,P<0.01),the distribution of the rest of the genotypes and alleles between cases and controls had no significant difference(P>0.05).Conclusion: LEPR gene Gln223Arg polymorphism is related to type 2 diabetes in Heilongjiang Han na-tionality.LEPR gene may be a related gene of type 2 diabetes in Chinese.更多还原