【摘要】 目的:观察麦冬不同提取物对过氧化氢诱导的人脐静脉内皮细胞(HUVEC)间黏附分子-1(ICAM-1)和VEGF、Bcl-2表达的影响。方法:体外培养HUVEC,用过氧化氢(H202)制造HUVEC损伤模型。以四甲基偶氮唑蓝(MTT)比色法检测细胞存活数量,用流式细胞仪检测HUVEC表面ICAM-1的表达量;免疫细胞化学方法检测HUVEC的VEGF、Bcl-2的分布情况。结果:模型组较正常对照组细胞增殖活性明显降低(P<0.01)。与模型组相比,经麦冬水提物、正丁醇提取物处理组细胞增殖活性明显增加(P<0.05,P<0.01)。流式细胞仪检测显示正丁醇提取物可降低过氧化氢增加的ICAM-1基因的表达。Bcl-2的表达,模型组明显低于正常对照组,而正丁醇组表达明显高于模型组(P<0.01)。VEGF的表达,模型组明显高于正常对照组,麦冬水提物、正丁醇提取物处理组高于模型组(P<0.05,P<0.01)。结论:麦冬提取物具有抗凋亡、促增殖、降低细胞间黏附分子-1表达的作用,尤以正丁醇提取物效果更为显著。更多还原

【Abstract】 Objective: To investigate the influence of different Ophiopogon Japonicus extracts on expression of intercellular adhe-sion molecule-1(ICAM-1) and VEGF,Bcl-2 in damaged human umbilical venous endothelial cell(HUVEC) induced by hydrogen per-oxide.Methods: HUVECs were cultured in vitro,and HUVEC damage model was induced by using hydrogen peroxide(H202).Blue methyl thiazolyl tetrazolium(MTT) method was used to detect the number of survival cells,the expression of ICAM-1 on the surface of HUVEC was analyzed by flow cytometry and immunocytochemical method was used to detect the distribution of VEGF,Bcl-2 in HUVEC.Results: Compared with the normal group,the cell proliferate activity of model group was significantly decreased(P <0.01),while after being treated by water extract and butanol extract from Ophiopogon Japonicus,it was significantly increased compared with the model group(P <0.05,P <0.01).The measurement of flow cytometry showed that the n-butanol extract could reduce the increased gene expression of ICAM-1 caused by hydrogen peroxide.For Bcl-2 expression,the model group was much less than the normal control group,and Ophiopogon Japonicus extract group was significantly higher than the model group(P <0.01).For VEGF expression,the mod-el group was much higher than the normal group,and aqueous extract and n-butanol extract of Ophiopogon Japonicus group was higher than the model group(P<0.05,P <0.01).Conclusion: Ophiopogon Japonicus extract,especially the extract by n-Butanol could be used for anti-apoptotic,promoting proliferation,reducing expression of ICAM-1.更多还原