【摘要】 目的:研究淫羊藿苷体外对致宫颈癌TC-1细胞的增殖抑制及促凋亡作用。方法:利用细胞培养,用不同浓度的淫羊藿苷在一定的时间处理致宫颈癌TC-1细胞,光学显微镜直接观察药物对细胞的作用;MTT法检测淫羊藿苷对TC-1细胞的增殖抑制作用;Dapi核染色、Annexinv-FITC/PI流式细胞学检测细胞凋亡。结果:淫羊藿苷对TC-1细胞有显著的抑制作用,且呈时间、剂量依赖,20μg/ml作用72小时后,细胞抑制率达99%;DAPI核染色和流式细胞术检测可发现典型细胞凋亡特征。结论:淫羊藿苷对TC-1细胞增殖有抑制和促凋亡作用,并呈时间浓度依赖性。更多还原

【Abstract】 Objective:To investigate the effect of icariin on proliferation and apoptosis.of TC-1 cells Methods:Based on the cul-ture of TC-1 cells in vitro,morphological changes of icariin-treated cells were detected by Optical microscope;The effect of icariin on growth of TC-1 cells was evaluated by MTT assay;DAPI stainin and flow cytometry(Annexin V-FITC/PI) were used to determine the apoptosis.Results:Optical microscope and MTT assay showed that the icariin had significant antiproliferative effect on TC-1 cells in a dose-and time-dependent manner significantly.When concentration of the icariin was 20 μg/ml,acting for 72 h,the inhibition rate of TC-1 cell growth reached to 99%;Features of apoptosis can be found by DAPI nuclear staining and flow cytometry.Conclusions:Icariin has a significantly effect on inhibiting proliferation and inducing apoptosis of TC-1 cells which was concentration and time dependence.更多还原