【摘要】 采用不同浓度的PEG-6000溶液模拟干旱胁迫,设置对照(CK)、轻度干旱胁迫(T1)、中度干旱胁迫(T2)、重度干旱胁迫(T3),研究了沙枣根茎叶的生长特性、可溶性蛋白、叶片光合色素对干旱胁迫的响应。结果表明:(1)随着干旱胁迫程度的加重,沙枣根长、株高,叶片数才开始受到影响,T2胁迫下,沙枣的根长、株高先受到影响,叶片数和CK差异不显著;T3胁迫下,沙枣根长受影响最大,株高其次,叶片数受影响最小。(2)T2胁迫下,沙枣的根、茎鲜(干)重先受到影响,叶鲜(干)和CK差异不显著;T3胁迫下,沙枣根鲜(干)重受影响最大,茎鲜(干)其次,叶鲜(干)受影响最小。(3)沙枣叶绿素a含量、叶绿素b含量、叶绿素a+b总含量、类胡萝卜素含量等均随着干旱胁迫的加剧先升高后下降,叶绿素a/b变化趋势相反,叶绿素b对干旱胁迫的反应较叶绿素a敏感。(4)在T1胁迫下,沙枣根中的可溶性蛋白高出CK18.6%,T2,T3胁迫下,根中可溶性蛋白分别低于CK 13.9%,29.1%;在T1胁迫下,沙枣茎中的可溶性蛋白和CK差异不显著,T2,T3胁迫下,沙枣茎中可溶性蛋白分别高出CK 17.2%,41.9%;叶片中的可溶性蛋白只有在T3胁迫下,显著高于CK 24.3%。更多还原

【Abstract】 PEG-6000 solutions with different concentrations were used to simulate drought stress.Four treatments were applied: control(CK),light stress(T1),moderate stress(T2) and heavy stress(T3) to investigate the effects of drought stress on photosynthetic pigment and root,stem and leaf growth characteristics of Elaeagnus angustifolia.The results show that root length,plant height,and leaf number of the plants exhibited the effects of increasing stress.In comparison of CK,root length,and plant height of T2 except for leaf numbers were significantly different.In T3,root length,plant height,leave number,respectively.In T2,fresh and dry weights of root and stem,except for leaf,indicated influences of draught stress in comparison of CK.Similarly,fresh and dry weights of root,stem,and leaf indicated strongest,moderate and least responses to the stress.Along with the increasing degree of drought stress,the contents of chlorophyll a,contents of chlorophyll b,contents of total chlorophyll,and contents of carotinoid Elaeagnus angustifolia increased first and then decreased,however,of the ratios of chlorophyll a/b showed opposite tendency.The response of chlorophyll a to drought stress is more sensitive than chlorophyll b.In comparison of CK,soluble proteins of the roots of T1 were 18.6% higher,but that of T2 and T3 were 13.9% and 29.1% lower,respectively.No significant difference was found between stem soluble proteins of T1 and CK,however,stem soluble protein of T2 and T3 was 17.2% and 41.9% higher than that of CK.T3 is the only treatment which leaf soluble proteins were significantly higher than CK(24.3%).更多还原