【摘要】 以清除DPPH自由基的能力为指标,研究罗非鱼皮色素抗氧化成分的提取工艺,探讨了提取物的分离纯化和抗氧化性能。结果表明,用柠檬酸预处理鱼皮,以甲醇为溶剂提取鱼皮色素提取率及其对DPPH自由基的清除率最高。在树脂吸附分离过程中,鱼皮色素在40 min内达到吸附平衡;吸附效率随样品浓度的增大而降低;丙酮洗脱鱼皮色素的解吸率最高;洗脱时间在20 min内达到解吸平衡;洗脱料液比为1∶2(mL/mL)时效果最优;上样流速在0.10 mL/min~0.20 mL/min范围内吸附效率达85%以上;解吸速度在0.15 mL/min~0.40 mL/min范围内解吸效率可以达到90%以上。鱼皮色素经树脂分离后,清除DPPH自由基的能力显著提高。X-5树脂对色素活性成分具有较强的吸附分离能力,有望应用于鱼皮色素中天然抗氧化成分的分离纯化。更多还原

【Abstract】 The ability of scavenging free DPPH radical was used to study the extraction technology of antioxidant components of tilapia skin pigment,discussed the separate purification of extract and the ability of antioxidant.Consequently,it was found that the highest extraction rate of skin pigment and scavenging rate of DPPH free radical were predominant produced on the treatment with citric acid after extracting in the methanol solution.In the resin adsorption process,the adsorption equilibrium achieved within 40min and the adsorption efficiency decreased with the increase of sample concentration.Followed by eluting the adsorbed resin with acetone,the best desorption efficiency of skin pigment in comparison with the total practical samples was obtained.The desorption equilibrium achieved within 20 min.Eluting liquid ratio of 1∶2(mL/mL) had the best effect.Adsorption efficiency could reach more than 85 % at a sample flow rate of 0.10 mL/min-0.20 mL/min.Desorption efficiency could achieved within 90 % at desorption rate 0.15 mL/min-0.40 mL/min,the ability of DPPH radical scavenging activity increased obviously.It can be expected for practical use to separate and purify of natural anti-oxidation component in fish skin pigment because X-5 resin mentioned above had strong adsorption and separation capacity for pigment active constituent.更多还原