【摘要】 运用气相色谱质谱联用方法,分析了鲢鱼、罗非鱼和草鱼3种淡水鱼脂肪的脂肪酸组成。淡水鱼脂肪经氯仿/甲醇(体积比=2∶1)提取后,用三氟化硼甲醇溶液衍生,衍生物用气相色谱质谱法分析。结果显示,3种淡水鱼脂肪多不饱和脂脂酸(PUFA)含量为29.26%～41.49%,主要有亚油酸(C18∶2)、亚麻酸(C18∶3)、花生四烯酸(C20∶4)、二十二碳五烯酸(C20∶5,EPA)和二十二碳六烯酸(C22∶6,DHA);单不饱和脂肪酸(MUFA)的含量为26.46%～39.60%,主要有棕榈油酸(C16∶1)和油酸(C18∶1);饱和脂肪酸(SFA)的含量为23.35%～37.05%,主要有肉豆蔻酸(C14∶0)、棕榈酸(C16∶0)和硬脂酸(C18∶0)。3种淡水鱼脂肪SFA∶MUFA∶PUFA的比值,鲢鱼为0.56∶0.84∶1;罗非鱼为1.07∶1.35∶1;草鱼为1.02∶0.72∶1。鲢鱼、罗非鱼和草鱼脂肪的n-6/n-3脂肪酸比值分别为1.16,2.46和1.98。3种淡水鱼脂肪具有较高的营养和开发价值。更多还原

【Abstract】 The fatty acids in the oil of three freshwater fishes,namely silver carp,tilapia,and grass carp were evaluated by gas chromatography-mass spectrometry.The fatty acid in the fishes were exacted with Chloroform-methanol(2∶1,volume ratio) and Derivatived with BF3-methanol,than analyzed by GC/MS.The result showed that polyunsaturated fatty acids were present at 29.26 %-41.49 %,the compositions mainly were linoleic acid(C18∶2),linoleic acid(C18∶3),arachidonic acid(C20∶4),docosapentenoic acid(C20∶5,EPA)and docosahexaenoic acid(C22∶6,DHA);monounsaturated fatty acids were present at 26.46 %-39.60 %,the compositions mainly were palmitoleic acid(C16∶1) and oleic acid(C18∶1);saturated fatty acids were present at 23.35 %-37.05 %,the compositions mainly were myristic acid(C14∶0),palmic acid(C16∶0) and stearic acid(C18∶0).The fatty acid ratio of SFA∶MUFA∶PUFA in the fishes oil,was 0.56∶0.84∶1 in silver carp,1.07∶1.35∶1 in tilapia and 1.02∶0.72∶1 in grass carp.The n-6/n-3 fatty acids ratio in the silver carp,tilapia,and grass carp was 1.16,2.46 and 1.98,respectively.It indicated that the oil of three freshwater fishes is benefic to health and should be exploited.更多还原