【摘要】 研究竹黄无性型菌株ZH-5-1液体发酵菌丝体中竹红菌素的稳定性及抗肿瘤活性。采用分光光度法测定竹红菌素溶液的吸光度以考察竹红菌素稳定性;以中国仓鼠卵巢肿瘤细胞(CHO细胞)株、人子宫颈癌细胞HeLa、HL-60人白血病细胞株和A-549人肺腺癌细胞株为模型,用Resazurin法检测液体发酵竹红菌素的抗肿瘤活性。结果显示:液体发酵竹红菌素在酸性条件下表现稳定;对热、光、H2O2、亚硫酸钠的稳定性良好;低浓度的金属离子不会影响竹红菌素稳定性,在所有供试离子中Mn2+、Cu2+、Fe2+对竹红菌素稳定性的破坏作用较大。液体发酵竹红菌素样品对4种供试肿瘤细胞株均具有生长抑制活性,对CHO、HL-60、A-549肿瘤细胞的抑制作用表现出良好的剂量-效应关系,其IC50分别为0.47、0.11、0.22mg/mL。总之,液体发酵竹红菌素是一种稳定的且具有抗肿瘤活性的天然产物,具有广阔的应用空间。更多还原

【Abstract】 The stability of hypocrellin from the liquid-cultured mycelia of a strain of anamorph(ZH-5-1) from Shiraia bambusicola was evaluated based on changes in the spectrophotometric absorbance of hypocrellin solutions.The anti-tumor activity was tested by Resazurin assay using CHO,HeLa,HL-60 and A-549 cells.The results showed that the hypocrellin was stable in acidic condition and had good thermal and light stability.Commonly used oxidant H2O2 and reducing agent Na2SO3 had no obvious effect on hypocrellin stability.The stability of the hypocrellin could not be influenced by low concentrations of metal ions,but among all the tested metal ions,Mn2+,Cu2+ and Fe2+ had more destructive effect.The hypocrellin inhibited the growth of all the four tested tumor cells.Hypocrellin inhibition on the growth of CHO,HL-60,and A-549 cells was in dose-dependent manner with an IC50 of 0.47,0.11 mg/mL and 0.22 mg/mL,respectively.In conclusion,mycelium hypocrellin of strain ZH-5-1 is a stable natural product with anti-tumor activity and thus will have potentially promising applications in practice.更多还原