【摘要】 目的:建立常山胡柚3种主要黄酮类物质含量的测定方法,并比较常山胡柚中皮、肉、籽中该3种黄酮类物质含量的差异。方法:采用反相高效液相色谱法分离检测胡柚中柚皮苷、橙皮苷、新橙皮苷的含量;流动相为乙腈-水(23:77,V/V),柱温25℃,流速1.0mL/min,检测波长283nm。结果:柚皮苷在3.22～1030μg/mL、橙皮苷在0.59～380μg/mL、新橙皮苷在0.077～990μg/mL范围内,黄酮类物质的质量浓度与峰面积呈良好的线性关系;柚皮苷、橙皮苷、新橙皮苷的平均回收率分别为98.43%(RSD=4.07%)、98.70%(RSD=4.30%)、99.90%(RSD=3.47%);并以柚皮苷、橙皮苷和新橙皮苷为标准比较胡柚副产物皮、籽与胡柚肉含量的差异,初步得出皮>肉>籽。结论:该方法简便、准确、可靠、重复性好,可用于常山胡柚的质量控制。更多还原

【Abstract】 Objective: To establish a method for determining naringin,hesperidin,and neohesperindin in Citrus paradisi cv.Changshan Huyou fruit and compare their contents in different parts such as peel,pulp and seeds.Methods: The contents of naringin,hesperidin and neohesperindin were determined by a reversed-phase HPLC method.Methanol extracts of samples and the standard substances were separated on a YMC C18 column(4.6 mm × 250 mm,5μm) using a mobile phase made up of acetonitrile and water(23:77,V/V) at a flow rate of 1 mL/min and detected by a UV detector at a wavelength of 283 nm.The column temperature was set at 25 ℃.Results: The calibration curves were linear in the range of 3.22-1030μg/mL for naringin,0.59-380μg/mL for hesperidin,and 0.077-990μg/m for neohesperindin,respectively.The average recoveries of naringin,hesperidin and neohesperindin were 98.43%(RSD = 4.07%),98.70%(RSD = 4.30%) and 99.90%(RSD = 3.47%),respectively.The contents of naringin,hesperidin,neohesperindin were the highest in the peel and the lowest in the seeds of Citrus paradisi cv.Changshan Huyou fruit.Conclusions: This method is simple,accurate,reliable and reproducible and can therefore be used for the quality control of Citrus paradisi cv.Changshan Huyou fruit.更多还原