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食用菌中氨基酸与铜离子配合工艺优化
Optimization of Chelation of Amino Acids from Shiitake Mushroom with Cupper Ion
【摘要】 以香菇为原料,采用酸水解提取法提取出复合氨基酸,并用氨基酸树脂对提取液中复合氨基酸进行纯化。对复合氨基酸与铜离子配合反应及产物进行初步的探讨研究。确定最佳的反应条件为反应温度70℃、反应时间70min、pH11.0,在此条件下,铜离子:氨基酸配合比为1:2(摩尔数比)。采用红外光谱对产物进行表征,得到了配合物的稳定常数。
【Abstract】 The chelation between amino acids from shiitake mushroom,extracted by acid hydrolysis and purified by amino acid resin adsorption,and cupper ion was preliminarily investigated.Using orthogonal array design,the optimal chelation conditions were determined to be reaction at 70 ℃ for 70 min at pH 11.0 and a molar ratio between cupper ion and amino acids of 1:2.The obtained product was characterized by infrared spectroscopy and its stability constant was also determined.
【基金】 黑龙江省自然科学基金面上项目(B200906)
- 【文献出处】 食品科学 ,Food Science , 编辑部邮箱 ,2011年18期
- 【分类号】TS201.2
- 【被引频次】1
- 【下载频次】142