【摘要】 采用部分析因试验设计(FFD)、中心组合实验设计(CCD)和响应面分析法(RSM)对桔青霉产核酸酶P1培养基进行优化研究。FFD结果表明:硫酸锌、酵母粉、复合磷酸盐3个因素显著影响桔青霉发酵产核酸酶P1,利用最陡爬坡实验(SAD)使得3个显著因素的水平取值逼近最大响应区域,通过CCD和RSM确定了3个显著性因素的最优水平,得到桔青霉发酵产核酸酶P1的最优培养基配方:葡萄糖4%、酵母粉0.681%、玉米浆0.4%、黄豆饼粉0.45%、硫酸锌0.042%、硫酸镁0.06%、氯化钙0.06%、复合磷酸盐(m(磷酸二氢钾):m(磷酸氢二钾)=1:1)0.068%。验证实验表明,优化后的培养基发酵酶活力达到563U/mL,比原培养基(202U/mL)提高了64%。更多还原

【Abstract】 Fractional factorial design(FFD),central composite design(CCD) and response surface methodology(RSM) were used to optimize the culture medium for nuclease P1 production by Penicillium citrinum.The results of fractional factorial design showed that ZnSO4,yeast extract and composite phosphate had a significant effect on nuclease P1 production.The steepest ascent design(SAD) made three significant factors approach the maximum response area.Three significant factors were optimized by CCD and RSM.The optimized culture medium was composed of 4% glucose,0.681% yeast extract,0.4% corn syrup,0.45% soybean meal,0.042% ZnSO4,0.06% MgSO4,0.06% CaCl2,and 0.068% composite phosphate(KH2PO4: K2HPO4 = 1:1).The activity of nuclease P1 from optimal culture medium was 563 U/mL,which revealed a 64% increase when compared with nuclease P1 activity(202 U/mL) from original fermentation medium.更多还原