【摘要】 目的:建立用邻二氮菲-Fe3+测定保健食品中水溶性成分抗氧化能力的方法。方法:用水相提取保健食品中的水溶性抗氧化成分,使其还原反应体系中的Fe3+,生成的亚铁离子与邻二氮菲反应成稳定的橙红色络合物,用分光光度计在536nm波长处测定吸光度。以水溶性VE为对照,由此计算样品水溶性成分中抗氧化(还原性)物质的多少。通过对方法线性关系、最低检测限、精密度、准确度等指标进行测定,验证方法的可行性。结果:检测Trolox在质量浓度0.66～10.53μmol/L范围内呈现良好的线性关系(R2=0.9999);最低检测下限为0.54μmol/L;平行性精密度为2.7%;重复性精密度为3.3%;5次重复加标回收率为(99.9±4.6)%。结论:邻二氮菲-Fe3+法可用于保健食品中水溶性成分抗氧化能力的检测。更多还原

【Abstract】 Objective: To establish a colorimetric method to determine the antioxidant capacity of water-soluble compounds in functional food based on the reaction between 1,10-2 phenanthroline and Fe3+ to form yellow complexes.Methods: The hydrophilic antioxidants in functional foods were extracted with pH 7.4 PBS buffer solution(75 mmol/L) and allowed to reduce Fe3+ to Fe2+.Fe2+ was exposed to 1,10-2 phenanthroline to form a stable Fe2+-phenanthroline complex.The absorbance of the complex was determined on a spectrophotometer at 536 nm and used to calculate the antioxidant capacity of water-soluble compounds in functional food using Trolox,a water soluble vitamin E analog,as the reference standard.The accuracy,linearity,repeatability,recovery rate and limit of detection(LOD) of this method were studied,and its feasibility was also verified.Results: This method showed a good linearity over the Trolox concentration of 0.66-10.53 μmol/L(R2 = 0.9999).The LOD was 0.54 μmol/L.The replicability and repeatability was 2.7% and 3.3% relative standard deviation(RSD),respectively.The mean spike recovery was(99.9±4.6)%(n = 5).Conclusion: This method is applicable to determine the antioxidant capacity of water-soluble compounds in functional food.更多还原