【摘要】 目的:以仿刺参纵肌多糖为对象,探讨其纯化方法,并对其抗氧化活性进行研究。方法:仿刺参纵肌经木瓜蛋白酶水解、三氯乙酸去蛋白、Sephacryl S-400凝胶柱层析,得到一种多糖,进行红外光谱、分子质量和硫酸基含量测定,并进行硫酸酯化及体外抗氧化实验。结果:红外光谱初步显示所得纯化多糖具有酸性糖胺聚糖结构,分子质量约为676kD,硫酸基含量为10.98%,取代度为0.285;硫酸酯化后硫酸基含量为17.43%,取代度为0.360;纯化多糖及酯化多糖都具有抗氧化活性。结论:仿刺参纵肌多糖随着硫酸基含量及取代度的提高,抗氧化活性逐渐增强。更多还原

【Abstract】 Objective: To separate and purify an antioxidant polysaccharide fraction from Apostichopus japonicus longitudinal muscle and to measure the abilities of the fraction and its sulfated counterpart to scavenge hydroxyl and superoxide anion free radicals.Methods: The separation and purification procedure was made up of papain hydrolysis,deprotenization with trichloroacetic acid,and Sephacryl S-400 gel filtration chromatography.The resulting fraction with higher free radical scavenging activity was selected for further studies including infrared spectral analysis and determination of molecular weight and sulfate content.Meanwhile,the fraction was sulfated,and its free radical scavenging activity was compared with that of its sulfated counterpart.Results: The obtained polysaccharide revealed an acidic glycosaminoglycan structure,whose relative molecular weight,sulfate content and substitution degree were 676 kD,10.98% and 0.285,respectively.Its sulfated counterpart had a sulfate content of 17.43% and a substitution degree of 0.360.Both of them had certain antioxidant activity to scavenge hydroxyl and superoxide anion free radicals,and the antioxidant activity of the sulfated polysaccharide was stronger.Conclusion: Sulfation can result in an increase in the sulfate content,substitution degree and antioxidant activity of the polysaccharide fraction with higher from Apostichopus japonicus longitudinal muscle.更多还原