【摘要】 目的:建立一种明胶掺假乳制品的快速定性方法。方法:采用硝酸汞溶液沉淀乳蛋白,进一步用苦味酸溶液检测明胶,通过敏感度测定试剂用量。结果:用1.4g/100mL硝酸汞溶液沉淀乳蛋白的最佳用量为乳品体积的1/2;用苦味酸检测明胶的敏感度为30mg/100mL。结论:该方法快速、方便,灵敏度高,可做成试剂盒,满足实际样品检测要求。更多还原

【Abstract】 Objective:To develop a method for the rapid qualitification of glutin-adulterated milk. Methods:For the precipitation of milk proteins,Hg(NO3)2 solution was used,and the supernatant resulting from centrifugation was then added with saturated picric acid solution. After thorough mixing,whether or not the reaction system becomes turbid was identified by comparison with a negative control. The sensitivity of the established method was evaluated by using simulated adulterated milk samples with varying amounts of glutin. Meanwhile,Hg(NO3)2 solution amount and centrifugation conditions were optimized. Results:Addition of a half volume of 1.4 g/100 mL Hg(NO3)2 solution to milk samples provided optimum precipitation of milk proteins. The detection sensitivity of the method for glutin was 300 mg/100 mL. Conclusion:This method is rapid,convenient and highly sensitive,and has the potential to be developed into reagent kit products,which can meet the requirements for detection of real samples.更多还原