【摘要】 目的:研究马齿苋正丁醇提取物对D-半乳糖致衰老小鼠的学习记忆能力及其作用机制。方法:取小鼠随机分为正常组、模型组、马齿苋正丁醇提取物(0.5、0.25g/(kg·d))组。除正常组颈部皮下注射生理盐水外,其他各组均皮下注射D-半乳糖(1g/(kg·d)),注射后每天下午灌胃给药,连续造模42d。采用避暗和水迷宫法测定衰老小鼠学习记忆能力,同时测定小鼠脑组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果:马齿苋正丁醇提取物组均可提高衰老小鼠学习记忆能力,并明显降低脑组织MDA含量,提高SOD和GSH-Px的活性。结论:马齿苋正丁醇提取物能明显改善衰老小鼠学习记忆障碍,其机制可能与提高SOD、GSH-Px活性,降低MDA含量有关。更多还原

【Abstract】 Objective:To study the effects of n-butanol extracts from Portulaca oleracea on the learning and memory performances of senile mice induced by D-gal and its mechanisms. Method:The mice were randomly divided into 3 groups:control group,model group and the n-butanol extracts(0.5 g/(kg·d) and 0.25 g/(kg·d) ) groups. The control group was given hypodermic injection of isotonic NaCl in the transverse part while the other groups successively administered with D-gal(1 g/(kg·d) ) . After injection of medicine in the afternoon of every day model animals were obtained after 42 d of treatment. Learning and memory performances were measured by step-through test and water maze test and the amount of MDA in mouse brain tissue and the activities of SOD and GSH-Px were determined. Results:Portulaca oleracea n-butanol extracts could improve the learning and memory performances of mice at both 0.5 g/(kg·d) and 0.25 g/(kg·d) ,and substantially reduce contents of MDA in brain tissues and improve activities of SOD and GSH-Px. Conclusion:Portulaca oleracea n-butanol extracts can markedly improve learning and memory performances of senile mice. The mechanisms of effects may be related to the improvement of SOD and GSH-Px activities and the decrease of MDA.更多还原