【摘要】 目的:利用响应面法对刺槐花多酚的提取工艺进行优化。方法:在单因素试验的基础上,选择温度、超声功率、超声时间、液料比为自变量,多酚提取得率为响应值,利用响应面分析法,研究各自变量交互作用及其对多酚得率的影响,模拟得到二次多项式回归方程的预测模型。结果:刺槐多酚类化合物的提取工艺为温度35℃、超声功率110W、超声时间36min、液料比20:1(mL/g)。结论:在最佳工艺条件下多酚得率为7.891%。更多还原

【Abstract】 Objective:The optimization of the extraction process of phenolic compounds from Robinia pseudoacacia L.flowers under ultrasound assistance was achieved by response surface methodology to maximize phenolic extraction yield.Methods:On the basis of single-factor experiments,Box-Behnken central composite design(CCD)involving four variables at three levels was utilized to set up a quadratic polynomial regression equation for phenolic extraction yield as function of extraction temperature,ultrasound power,length of ultrasound treatment time and material/liquid ratio,and the pairwise interactive effects of the above four variables on their function were investigated by response surface analysis.Results:The optimum conditions for extracting phenolic compounds from Robinia pseudoacacia L.flowers were found to be:36 min ultrasound treatment at 110 W power for extracting the raw material with a 20-fold volume of 60%aqueous ethanol solution at 35℃.Under these optimum conditions,the yield of polyphenols was 7.891%.更多还原