【摘要】 比较了7种大孔吸附树脂对磷脂酶Lecitase誖Ultra的固定化效果,对固定化条件进行了研究。结果表明,极性树脂DA201为该酶的最适固定化载体,在室温下,以pH7.0,0.01mol/L的Tris-HCl缓冲液为媒介,加酶量30mg/g树脂,采用正硅酸乙酯对经真空干燥的固定化酶进行涂层处理,通过电镜扫描发现其涂层效果较好。在最适条件下得到的固定化酶活力为1250～1300U/g,连续操作5次后酶活保存率为55%。与游离酶比较,固定化磷脂酶Lecitase誖Ultra的热稳定性、pH稳定性均有一定程度的提高。更多还原

【Abstract】 Seven kinds of macroporous resin were employed to immobilize phospholipase Lecitase Ultra,and the immobilization conditions were studied.The results indicated that DA201 was the best carrier.The optimum operation conditions were:under room temperature,pH 7.0 Tris-HCl(0.01mol/L) ,enzyme dosage 30mg/g resin.Tetraethyl orthosilicate was then used as the coating reagent for immobilized enzyme by the vacuum drying,which showed an effective coating effect through scanning electron microscope.Under optimized conditions,the immobilized Lecitase Ultra with enzyme activity of 1250～1300U/g could be obtained,and it could remain 55% enzyme activity after continuous operation for 5 times.Compared with free Lecitase Ultra,there was a certain extent improvement for the thermal stability and pH stability of immobilized Lecitase Ultra.更多还原