【摘要】 对菊芋叶片中绿原酸的提取工艺条件进行研究,探讨了提取方法、提取试剂、提取温度、时间、固液比等因素对绿原酸含量的影响。在单因素实验的基础上,采用正交实验法优化了菊芋叶片绿原酸的提取工艺。最佳提取工艺为:回流提取法,提取试剂为70%甲醇,提取温度80℃,提取时间2.5h,固液比1∶25,测得的绿原酸平均含量为1.6375mg/g。研究结果可为利用菊芋叶片工业化生产绿原酸提供科学依据。更多还原

【Abstract】 The extraction technology of chlorogenic acid from Helianthus tuberosus leaves was studied by discussing the effect of extraction methods,extraction reagents,extraction temperatures and times,the ratio of material/solution on the content of chlorogenic acid.Based on the single factor experiments,the extraction technology of chlorogenic acid from Helianthus tuberosus leaves was optimized by orthogonal design.The optimal processes conditions were:reflux extraction,70% methanol concentrations as extraction reagent,80℃ extraction temperatures,2.5h extraction time and the ratio of material/solution was 1∶25.Under the condition above,chlorogenic acid content was 1.6375mg/g.The results provided scientific basis for chlorogenic acid extraction from Helianthus tuberosus leaves.更多还原